Journal: Molecular Medicine Reports

Article Title: Exploring the anti‑oxidative mechanisms of Rhodiola rosea in ameliorating myocardial fibrosis through network pharmacology and in vitro experiments

doi: 10.3892/mmr.2024.13338

Figure Lengend Snippet: SAL improves myocardial fibrosis through multiple targets. (A) The level of myocardial fibrosis marker COL III by immunofluorescence (magnification, ×20; scale bar, 100 µm) (B) The mRNA levels of IL6, MMP9, AKT1, ALB and CASP3 by reverse transcription-quantitative PCR. *P<0.05, **P<0.01 , ***P<0.001. SAL, salidroside; COL, collagen; AKT1, AKT serine/threonine kinase 1; ALB, albumin; CASP3, caspase-3.

Article Snippet: Then, the cells were incubated overnight at 4°C with 100 μl primary antibody against COL III (1:100; cat. no. A3795; ABclonal Biotech Co., Ltd.).

Techniques: Marker, Immunofluorescence, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/IJN.S466312

Figure Lengend Snippet: RCDs/UA@Lipo alleviated H 2 O 2 -induced oxidative stress and TGF-β1 induced fibrosis-specific markers separately. ( A ) Immunofluorescence staining images of intracellular ROS and superoxide detected by DCFH-DA probe. ( B ) mitochondrial membrane potential (ΔΨm) detected by JC-1 probe. ( C ) tendon fibrosis markers (COL I, COL III, α-SMA) of NIH3T3. ( D–H ) Semiquantitative analysis of the relative fluorescent intensity of ( A–C ) (n = 3). ( D and E ) Model group is H 2 O 2 group, ( F–H ) Model group is TGF-β1 group. Data are presented as mean ± SD; comparisons between the groups were performed one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: The fresh tissue sections were incubated with rabbit anti-rat antibodies against COL III (1:200; Cat#22734-1-AP, Proteintech, USA) and α-SMA (1:200; Cat#19245S, Cell Signaling Technology, USA) at 4°C overnight.

Techniques: Immunofluorescence, Staining, Membrane

Journal: International Journal of Nanomedicine

Article Title: Antioxidant Carbon Dots and Ursolic Acid Co-Encapsulated Liposomes Composite Hydrogel for Alleviating Adhesion Formation and Enhancing Tendon Healing in Tendon Injury

doi: 10.2147/IJN.S466312

Figure Lengend Snippet: RCDs/UA@Lipo-HAMA reduced adhesion of injured tendons at macroscopic and histological levels at various time points post-injury. ( A ) Schematic diagram of surgical procedures of the ATI. ( B ) Gross view of ATI. ( C )Representative images of immunofluorescence staining of tendon antioxidant (Nrf-2, HO-1) and anti-inflammatory markers (CD68, iNOS) at 2 weeks post-injury (n = 3). ( D ) Representative images of immunofluorescence staining of tendon antifibrosis (COL III, α-SMA) at 6 weeks post-injury (n = 3). ( E ) Representative images of immunohistochemical staining of tendon markers (Vimentin, MMP2, α-SMA) antifibrosis at 6 weeks post-injury (n = 4). ( F–N ) Semiquantitative analysis of expression level of tendon marker of ( C–E ), respectively. Data are presented as mean ± SD; comparisons between the groups were performed by one-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Article Snippet: The fresh tissue sections were incubated with rabbit anti-rat antibodies against COL III (1:200; Cat#22734-1-AP, Proteintech, USA) and α-SMA (1:200; Cat#19245S, Cell Signaling Technology, USA) at 4°C overnight.

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Expressing, Marker

Journal: Cells

Article Title: NSC828779 Alleviates Renal Tubulointerstitial Lesions Involving Interleukin-36 Signaling in Mice

doi: 10.3390/cells10113060

Figure Lengend Snippet: NSC828779 alleviates pathological changes in UUO mice. ( A , B ) H&E staining and scoring. ( C , D ) Masson trichrome staining and scoring of tubulointerstitial fibrotic area. IHC and scoring for F4/80 + ( E , F ), CD3 + ( G , H ), Col-III ( I , J ) and p-Smad2/3 ( K , L ). Original magnification, each 400×. Data are shown as means ± SEM of seven mice per group. The bar indicates 20 µm. Arrows indicate positive staining. The horizonal dashed line indicates the mean for sham control + NSC828779. NSC828779, a salicylanilide derivative. UUO, unilateral ureter obstruction; TILs, tubulointerstitial lesions. H&E staining, hematoxylin and eosin stain. Col-III, collagen III. # not detectable. * p < 0.05, ** p < 0.01, *** p < 0.005, **** p < 0.001, †††† p < 0.001 Day 7 versus Day14 of UUO + NSC828779, ‡ no significant difference Day 7 versus Day14 of UUO + NSC828779.

Article Snippet: For IHC analysis, renal tissue sections were stained with antibodies against F4/80 (Bio-Rad, Hercules, CA, USA), CD3 (Dako, Glostrup, Denmark), collagen (Col)-I, Col-III (Southern Biotech, Birmingham, AL, USA), p-Smad2/3 (Santa Cruz, CA, USA) or IL-36α (R&D Systems, Minneapolis, MN, USA).

Techniques: Staining, Control, H&E Stain